Journal: Journal of Neuroinflammation

Article Title: Sex-dependent effects of carbohydrate source and quantity on caspase-1 activity in the mouse central nervous system

doi: 10.1186/s12974-024-03140-5

Figure Lengend Snippet: Diet alters the male mouse microbiome. DNA was extracted from 26-week-old male mice and 16S ribosomal RNA gene amplicon sequencing performed. A Bray–Curtis Dissimilarity Index identified clusters of samples that were similar in composition. B Alpha diversity analysis was performed by calculating richness (ace, chao1, and observed richness), evenness (Pielou) and overall diversity (Shannon and Simpson). C Beta diversity was assessed by PCoA using the Bray–Curtis Dissimilarity Index at the ASV level and resultant values tested for significance with the experimental covariates using the PERMANOVA test. N = 8–10 per group. Statistical comparisons of bacterial abundance were conducted using the Phyloseq package and involved pairwise Chi-Squared Tests, and results were filtered based on a significance threshold of Benjamini-Hochberg adjusted p-value < 0.01

Article Snippet: Statistical comparisons of bacterial relative abundance were conducted using the Phyloseq package and involved pairwise Chi-Squared Tests, and results were filtered based on a significance threshold of Benjamini-Hochberg adjusted p-value < 0.01.

Techniques: Amplification, Sequencing